The Development of the Infant and Young Child

plates 16. 45s.). Edinburgh and London: E. & S. Livingstone, 1970. IT is now more than ten years since Dr. Paul introduced from the Tissue Culture Association's Summer School the idea, which is now well realised, that temporary or continuous multiplication of mammalian cells is practical laboratory politics. Since then his book has come to the fourth edition, and the unaltered arrangement of the book, following many advances, is a tribute to the partition of subjects in the earliest edition. The parts deal with cell structure, preparation of materials, special techniques and, finally, applications of the method. There are now four very useful appendices dealing with formulae for media, commercial sources of materials, standard cell lines, and nomenclature. Part one contains a necessary amount of theoretical information which is simply presented and kept well within bounds. Part two is full of practical advice, ranging from glass-cleaning to designing a tissue-culture laboratory, and will reassure the most timid of those who have previously been discouraged by imaginary difficulties. Part three deals with tissue culture, organ culture, cell culture and cloning and has a new chapter on large scale culture of cells. Part four has three chapters on a great vanety of applcations including virological techniques. All chapters have numerous and most apposite references to the literature of cell culture. Many line diagrams have been retained and the advantage to a novice of seeing where the carotid arteries of a bird may be found or what a "Cornwall" syringe is, need not be stressed. The books is indispensible to those in a tissue-culture laboratory and the readers will find a lucid practical account in which theory and history are kept in proper perspective. K.B.F.